Regulatory

Part:BBa_I748001:Design

Designed by: Kurt Whittemore   Group: iGEM07_Southern_Utah   (2007-10-26)


Putative Cyanide Nitrilase Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We isolated this segment of DNA from the Pseudomonas fluorescens PfO-1 genome using a PCR reaction.


Source

This section of DNA comes from the Pseudomonas fluorescens PfO-1 genome (NC_007492). The GeneID for the nitrilase gene that we working with is 3715134, and the protein ID for the nitrilase gene is YP_349417.1. We took the region 300 base pairs upstream of the nitrilase gene and 10 base pairs downstream into the gene.

References